Investigation of the Prevalence of vanA and vanB genes in vancomycin resistant enterococcus (VRE) by Taq Man real time PCR Assay
نویسندگان
چکیده
منابع مشابه
Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci
BACKGROUND Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with the Seeplex VRE ACE detection kit (Seeplex; Seegene, Korea), a conventional multiplex PCR assay. METHODS A chromogenic agar-based culture, in which pre-selected vancomycin-resista...
متن کاملDetection of both vanA & vanB genes in vanA phenotypes of Enterococci by Taq Man RT-PCR
Twenty seven isolates of vancomycin resistant Enterococci based on the disk diffusion and E- test have been screened; being found eight (0.3%) clinical isolates of vanA & vanB through Taq Man Real Time PCR assay. This study shows the presence of both vanA & vanB genotypes in vanA phenotypes clinical isolates in the three hospitals in Iran.
متن کاملEvaluation of the EVIGENE VRE detection kit for detection of vanA and vanB genes in vancomycin-resistant enterococci.
The aim of this study was to evaluate the performance of the EVIGENE VRE Detection kit and compare it with PCR, considered the gold standard for detection of vancomycin-resistant enterococci (VRE). The correlation between the MIC values of vancomycin and teicoplanin using the epsilon test was also determined. In the EVIGENE VRE Detection kit, DNA probes specific for bacterial target DNA sequenc...
متن کاملReal-time PCR surveillance of vanA for vancomycin-resistant Enterococcus faecium.
In Korea, the incidence of vancomycin-resistant enterococci (VRE) infection has recently been increasing, with a clinical isolation rate of up to 25%. We evaluated the clinical usefulness of real-time PCR for the detection of the vanA gene over conventional culture in patients confirmed as being VRE-colonized. During the evaluation stage, 100 cons...
متن کاملEpidemiologic evaluation of Vancomycin Resistant genes in Enterococcus spp. isolated from clinical samples
Background & Objectives: Isolation of vancomycin resistant Enterococcus from clinical samples is very important. The aim of this study was evaluation of phenotype and genotype of van genes in vancomycine resistant Enterococcus. Materials and Methods: 411 Enterococcus isolates were collected from selected Tehran’s hospitals between March 2004 and December 2007. The enterococcal isolates were ide...
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ژورنال
عنوان ژورنال: Journal of Microbiology and Infectious Diseases
سال: 2013
ISSN: 2146-3158,2146-9369
DOI: 10.5799/ahinjs.02.2013.04.0107